Journal: Neural Plasticity

Article Title: Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

doi: 10.1155/2016/3081939

Figure Lengend Snippet: Immunohistochemical double staining of BrdU, MAP2, GFAP, and GalC positive cells in the cerebral parenchyma of rats. (a–c) MAP2 − /BrdU + cells (solid arrow), MAP2 + /BrdU − cells (dotted arrow), and MAP2 + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (d–f) GalC − /BrdU + positive cells (solid arrow), GalC + /BrdU − cells (dotted arrow), and GalC + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (g–i) GFAP − /BrdU + cells (solid arrow), GFAP + /BrdU − cells (dotted arrow), and GFAP + /BrdU + cells (arrowhead) in NSCs, T3/NSCs, and GDNF-T3/NSCs group, respectively. (j) GFAP + cells (dotted arrow) in control group. (k) GFAP + cells (dotted arrow) in normal group. Bar (a–k) = 25 μ m.

Article Snippet: After a goat anti-mouse IgG secondary antibody (1 : 100; Wuhan Boster Biological Technology, China) was added for 30 minutes, alkaline phosphatase- (AP-) streptavidin (Boster) was incubated for 30 minutes, and 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride (BCIP/NBT, Boster) was then used as a chromogen for 10 min. After PBS washes, the sections were reincubated with primary antibody for rabbit ant-MAP2, GFAP, or GalC, followed by incubation with goat anti-rabbit IgG (1 : 100; Boster) and horseradish peroxidase- (HRP-) streptavidin (Boster).

Techniques: Immunohistochemical staining, Double Staining, Control

Journal: Neural Plasticity

Article Title: Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

doi: 10.1155/2016/3081939

Figure Lengend Snippet: Differentiation of NSCs, T3/NSCs, and GDNF-T3/NSCs in vivo (mean ± SD, n = 5).

Article Snippet: After a goat anti-mouse IgG secondary antibody (1 : 100; Wuhan Boster Biological Technology, China) was added for 30 minutes, alkaline phosphatase- (AP-) streptavidin (Boster) was incubated for 30 minutes, and 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium chloride (BCIP/NBT, Boster) was then used as a chromogen for 10 min. After PBS washes, the sections were reincubated with primary antibody for rabbit ant-MAP2, GFAP, or GalC, followed by incubation with goat anti-rabbit IgG (1 : 100; Boster) and horseradish peroxidase- (HRP-) streptavidin (Boster).

Techniques: In Vivo

Journal: Scientific Reports

Article Title: Stem cells in middle ear cholesteatoma contribute to its pathogenesis

doi: 10.1038/s41598-018-24616-4

Figure Lengend Snippet: Overexpression of the Toll-like receptor 4 (TLR4) in cholesteatoma tissue is conserved in the ME-CSCs and accompanied by expression of LCN2, TNF-α and A20. ( A ) Immunohistochemical staining of the cholesteatoma tissue and ACS showed increased expression of TLR4 on protein-level in the cholesteatoma tissue in comparison to the ACS. Scale bar: 50 µm. ( B ) Real time qPCR depicted significantly increased expression levels of the pro-inflammatory genes TNF-α and A20 in ME-CSCs and an increased response after the treatment with 100 ng/ml LPS for 6 h in comparison to the ACSCs (technical triplicates ***p < 0.001 was considered significant, One-way ANOVA, Bonferroni´s Multiple Comparison Test, confidence interval: 95%). ( C ) On the mRNA-level real time PCR analysis of biological triplicates revealed significantly increased expression of the TLR4 and the innate immune protein Lipocalin2 (LCN2) in the ME-CSCs compared to the ACSCs. (***p < 0.001, **p < 0.01 and *p < 0.05 was considered significant, unpaired t-test, two tailed, confidence interval: 95%).

Article Snippet: Primary antibodies used were mouse anti-Nestin 1:200 (Millipore), rabbit anti-S100B 1:100 (Dako) for stem cell detection already utilized in Hauser et al . . Additional primary antibodies were mouse anti-β-III-tubulin 1:100 (Promega), rabbit anti-MAP2 1:100 (Santa Cruz Biotechnology), mouse anti-TLR4 1:100 (Acris Antibodies GmbH), rabbit ant-β1-integrin (Sigma Aldrich), mouse anti-CK-14 1:200 (Millipore) and mouse anti-CK-18 1:800 (Cell Signaling Technology).

Techniques: Over Expression, Expressing, Immunohistochemical staining, Staining, Real-time Polymerase Chain Reaction, Two Tailed Test

Journal: Scientific Reports

Article Title: Stem cells in middle ear cholesteatoma contribute to its pathogenesis

doi: 10.1038/s41598-018-24616-4

Figure Lengend Snippet: Primer sequences.

Article Snippet: Primary antibodies used were mouse anti-Nestin 1:200 (Millipore), rabbit anti-S100B 1:100 (Dako) for stem cell detection already utilized in Hauser et al . . Additional primary antibodies were mouse anti-β-III-tubulin 1:100 (Promega), rabbit anti-MAP2 1:100 (Santa Cruz Biotechnology), mouse anti-TLR4 1:100 (Acris Antibodies GmbH), rabbit ant-β1-integrin (Sigma Aldrich), mouse anti-CK-14 1:200 (Millipore) and mouse anti-CK-18 1:800 (Cell Signaling Technology).

Techniques: Sequencing